Transformation of canola by chit33 gene towards improving resistance to Sclerotinia sclerotiorum
An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in canola (R line Hyola 308).Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens.The insertion of the transgene was verified by PCR and Clothing Southern VOLUME AND CURL MASCA